• Catalog Number:

  E1A7195A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15530

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.

• References:

  Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63. Chen H, et al. (2001) Biochemistry; 40(39): 11851-9. Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7. Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody.

Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody.