• Catalog Number:

  E1A8433B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human TIF-IA around the phosphorylation site of serine 649 (Q-P-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9NYV6

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  RNA polymerase I-specific transcription initiation factor RRN3; RRN3; transcription initiation factor IA

• Specificity Sensitivity:

  TIF-IA (Ab-649) antibody detects endogenous levels of total TIF-IA protein.

• References:

  Bodem J., EMBO Rep. 1:171-175(2000). Moorefield B., Proc. Natl. Acad. Sci. U.S.A. 97:4724-4729(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using TIF-IA (Ab-649) antibody.