• Catalog Number:

  E1A7182A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 33 (V-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  p53 (phospho-Ser33) antibody detects endogenous levels of p53 only when phosphorylated at serine 33.

• References:

  Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Latonen L, et al. (2001) Oncogene. 20(46): 6784-6793. Dumaz N, et al. (2001) Biochem J. 359(Pt 2): 459-464.

Western blot analysis of extracts from 293 cells using p53 (Ab-33) antibody (Line 1 and 2) and p53 (phospho- Ser33) antibody (Line 3 and 4).