Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
DARPP-32 (Ab-34) antibody detects endogenous levels of total DARPP-32 protein.
References:
Brene S., J. Neurosci. 14:985-998(1994).
El-Rifai W., Cancer Res. 62:4061-4064(2002).
Liu Q.-R., Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
Western blot analysis of extracts from COS-7 cells, treated with forskolin (40nM, 30mins), using DARPP-32 (Ab-34) antibody.