• Catalog Number:

  E1A7176A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of Serine10 (N-G-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P46527

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  p27Kip1 (Phospho-Ser10) Antibody detects endogenous levels of p27Kip1 only when phosphorylated at Serine 10.

• References:

  Le XF, et al. (2003) J Biol Chem. Connor MK, et al. (2003) Mol Biol Cell; 14(1): 201-13. McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28. Chopra S, et al. (2002) J Biol Chem; 277(36): 32413-6. Boehm M, et al. (2002) EMBO J; 21(13): 3390-401.

Western blot analysis using p27Kip1 (Phospho-Ser10) Antibody: Line1: The extracts from A2780 cells; Line2: The extracts from COLO205 cells; Line3: The extracts from COLO205 cells using p27Kip1 (Phospho-Ser10) Antibody preincubated with synthesized peptide.