Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage/Stability:
Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
class="eg_cn_title"Immunogen:
A phospho specific peptide corresponding to residues surrounding S139 ofhuman Histone H2A.x
Purification:
Affinity purification
Reactivity:
H M R
SwissProtNo:
P16104
Source:
Rabbit
Applications:
WB IHC
Alter Name:
H2AFX; Histone H2A.x;
References:
1. Yuan, J. et al. (2010) FEBS Lett 584, 3717-24. 2. Rogakou, E.P. et al. (1998) J Biol Chem 273, 5858-68. 3. Burma, S. et al. (2001) J Biol Chem 276, 42462-7. 4. Rogakou, E.P. et al. (1999) J Cell Biol 146, 905-16. 5. Mukherjee, B. et al. (2006) DNA Repair (Amst) 5, 575-90. 6. Solier, S. et al. (2009) Mol Cell Biol 29, 68-82. 7. Lu, C. et al. (2006) Mol Cell 23, 121-32. 8. Lu, C. et al. (2008) FEBS Lett 582, 2703-8. 9. Cook, P.J. et al. (2009) Nature 458, 591-6. 10. Xiao, A. et al. (2009) Nature 457, 57-62.
WB 1:500 - 1:2000
IHC 1:50- 1:200
Western blot analysis on Jurkat cell lysatesusing anti-Phospho-H2A.x (pS139).Cells wereeitheruntreated or treated with etoposide.