• Catalog Number:

  E1A7166A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 337 (R-K-SP-D-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19838

• Applications:

  WB: 1:200~1:500 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  NF-κB p105/p50 (phospho-Ser337) antibody detects endogenous levels of NF-κB p105/p50 only when phosphorylated at serine 337.

• References:

  Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p105/p50 (phospho-Ser337) antibody.

Western blot analysis of extracts from MDA-MB-435 cells using NF-κB p105/p50 (phospho-Ser337) antibody.