Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
CDC25C (Ab-216) antibody detects endogenous levels of total CDC25C protein.
References:
Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M., Int. J. Oncol. 17:1251-1258(2000).
Livingston R.J., Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
Western blot analysis of extracts from HeLa cells and Jurkat cells treated with UV (15mins), using CDC25C (Ab-216) antibody.