• Catalog Number:

  E1A1008A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of serine 725 (A-P-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P42229

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  MGF, MPF, Mammary gland factor, STA5A, STAT5, STAT5A, Signal transducer and activator of transcription 5A

• Specificity Sensitivity:

  STAT5A (Phospho-Ser725) antibody detects endogenous levels of STAT5A only when phosphorylated at serine 725.

• References:

  Hou J., Immunity 2:321-329(1995). Litterst C.M., J. Biol. Chem. 278:45340-45351(2003). Martens N., J. Biol. Chem. 280:13817-13823(2005). - ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Ser726; Rat: Ser725

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using STAT5A (Phospho-Ser725) antibody. Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using STAT5A (Phospho-Ser725) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using STAT5A (Phospho-Ser725) antibody.