• Catalog Number:

  E1A7129A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SAPK/JNK around the phosphorylation site of tyrosine 185 (T-P-YP-V-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P45983/P45984/P53779

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK-46; JNK1-alpha-2; kinase JNK1; MAPK8; Mitogen-activated protein kinase 8; MK08; p54 gamma; PRKM8; SAPK gamma; Stress-activated protein kinase JNK1

• Specificity Sensitivity:

  SAPK/JNK (Phospho-Tyr185) antibody detects endogenous levels of SAPK/JNK only when phosphorylated at tyrosine 185.

• References:

  Derijard B., Cell 76:1025-1037(1994). Gupta S., EMBO J. 15:2760-2770(1996). Derijard B., Science 267:682-685(1995).

• All Sites:

  Human: Tyr185; Mouse: Tyr185; Rat: Tyr185

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SAPK/JNK (Phospho-Tyr185) antibody. Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using SAPK/JNK (Phospho-Tyr185) antibody.