• Catalog Number:

  E1A1149A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451 (L-G-TP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P98177

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  AFX; AFX1; Afxh; forkhead box protein O4; forkhead domain AFX1; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 (AFX1)

• Specificity Sensitivity:

  FOXO4 (Phospho-Thr451) antibody detects endogenous levels of FOXO4 only when phosphorylated at threonine 451.

• References:

  Peters U., Hum. Genet. 100:569-572(1997). Borkhardt A., Oncogene 14:195-202(1997). Yang Z., J. Biol. Chem. 277:8068-8075(2002).

• All Sites:

  Human: Thr451; Mouse: Thr452

Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 5mins), using FOXO4 (Phospho-Thr451) antibody.