• Catalog Number:

  E1A7111A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04792

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  HspB1; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein

• Specificity Sensitivity:

  HSP27 (Phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.

• References:

  J Landry, et al. (1992) J. Biol. Chem; 267: 794-803. Sita Somara and Khalil N. Bitar Am J Physiol, (2004) Cell Physiol; 286: C1290-C1301. Elke Butt, et al. (2001) J. Biol. Chem; 276: 7108-7113. Rainer Benndorf, et al. (2001) J. Biol. Chem; 276: 26753-26761.

• All Sites:

  Human: Ser78

Western blot analysis of extracts from Hela cells, untreated or treated with Ca2+, using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho- Ser78) antibody (Line 3 and 4).