• Catalog Number:

  E1A7046A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 73 (L-A-SP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05412

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  c-Jun (phospho-Ser73) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 73.

• References:

  Sevilla A, et al. (2004) Oncogene.23 (55): 8950-8958. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. Binetruy B, et al. (1991) Nature. 351:122-127. Smeal T, et al. (1991) Nature. 354: 494-496. Derijard B, et al. (1994) Cell. 76:1025-1037.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (phospho-Ser73) antibody.

Western blot analysis of extracts from HeLa cells treated with UV, using c-Jun (phospho-Ser73) antibody.