• Catalog Number:

  E1A7105A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of Tyrosine 877 (T-E-YP-H-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04626

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  HER2(Phospho-Tyr877) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine 877.

• References:

  Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444. Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using HER2 (Phospho-Tyr877) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis of extracts from MDA-MB-231 cells treated or untreated with EGF using HER2 (Ab-877) Antibody and HER2 (Phospho- Tyr877) Antibody.