• Catalog Number:

  E1A7077A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 118 (Q-L-SP-P-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P03372

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  Estrogen Receptor-α (phospho-Ser118) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 118.

• References:

  Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014. Masuhiro Y, et al. (2005). Proc Natl Acad Sci U S A.102 (23): 8126-8131. Pentecost BT, et al. (2005). Mol Cell Endocrinol.238 (1-2): 9-25. Park KJ, et al. (2005). Mol Cell.18 (1): 71-82.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (phospho-Ser118) antibody.

Western blot analysis of extracts from MCF7 cells treated with Estradiol, using Estrogen Receptor-α (phospho-Ser118) antibody.