• Catalog Number:

  E1A1129A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TNFRSF5 around the phosphorylation site of threonine 254 (Q-E-TP-L-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P25942

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  B-cell surface antigen CD40; Bp50; CD40 antigen; CD40L receptor; CDw40; TNR5; tumor necrosis factor receptor superfamily member 5

• Specificity Sensitivity:

  TNFRSF5 (Phospho-Thr254) antibody detects endogenous levels of TNFRSF5 only when phosphorylated at threonine 254.

• References:

  Stamenkovic I., EMBO J. 8:1403-1410(1989). Tone M., Proc. Natl. Acad. Sci. U.S.A. 98:1751-1756(2001). Deloukas P., Nature 414:865-871(2001).

• All Sites:

  Human: Thr254; Mouse: Thr255

Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using TNFRSF5 (Phospho-Thr254) antibody.