• Catalog Number:

  E1A7071A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176 (T-Q-SP-F-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29474

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  Constitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS

• Specificity Sensitivity:

  eNOS (phospho-Ser1176) antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176 .

• References:

  Yong Chool Boo, Am J Physiol Heart Circ Physiol, Nov 2002; 283: 1819. Christian Pott, Am J Physiol Cell Physiol, May 2006; 290: C1437 - C1445. Monica Montagnani, J. Biol. Chem., Aug 2001; 276: 30392 - 30398. Peter F. Mount, Am J Physiol Renal Physiol, Nov 2005; 289: F1103 - F1115.

• All Sites:

  Human: Ser1176; Mouse: Ser1175; Rat: Ser1175

Western blot analysis of extracts from Hela cells treated with Insulin (0.01U/ml, 15mins), using eNOS (phospho-Ser1176) antibody (Line 1 and 2).