• Catalog Number:

  E1A8459A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FHIT around the phosphorylation site of tyrosine 114 (S-I-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P49789

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  AP3A hydrolase; AP3AASE; Bis(5'-adenosyl)-triphosphatase; Bis(5'-adenosyl)-triphosphatease; Dinucleosidetriphosphatase; EC 3.6.1.29; Fragile histidine triad protein

• Specificity Sensitivity:

  FHIT (Phospho-Tyr114) antibody detects endogenous levels of FHIT only when phosphorylated at tyrosine 114.

• References:

  Ohta M., Cell 84:587-597(1996). Druck T., Cancer Res. 57:504-512(1997). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Tyr114

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using FHIT (Phospho-Tyr114) antibody. FHIT (Phospho-Tyr114) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.