• Catalog Number:

  E1A1210A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 226 (E-I-SP-D-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08238

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2, HSPCB, Heat shock protein HSP 90-beta

• Specificity Sensitivity:

  HSP90B (Phospho-Ser226) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 226.

• References:

  Rebbe N.F., Gene 53:235-245(1987). Rebbe N.F., J. Biol. Chem. 264:15006-15011(1989). Wiemann S., Genome Res. 11:422-435(2001).

• All Sites:

  Human: Ser226; Mouse: Ser226; Rat: Ser226

P-peptide - + Western blot analysis of extracts from A549 cells, treated with serum (20%, 15mins), using HSP90B (Phospho-Ser226) antibody. Immunofluorescence analysis of NIH/3T3 cells, using HSP90B (Phospho-Ser226) antibody.