• Catalog Number:

  E1A7067A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06730

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  Eukaryotic translation initiation factor 4E, IF4E, eIF-4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

• Specificity Sensitivity:

  elF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.

• References:

  Li BD, et al. (1998) Ann Surg; 227(5): 756-763. Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520. De Gregorio E, et al. (2001) RNA; 7(1): 106-113. Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461.

• All Sites:

  Human: Ser209; Mouse: Ser 209; Rat: Ser 209

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using elF4E (phospho- Ser209) antibody.

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with FBS, using elF4E (Ab-209) antibody (Line 1 and 2) and elF4E (Phospho- Ser209) antibody (Line 3 and 4).