• Catalog Number:

  E011184-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P68431

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

• Specificity Sensitivity:

  Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.

• References:

  Dai J, et al. (2005) Genes Dev 19(4): 472-488. Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (phospho-Ser10) antibody (E011184).

Western blot analysis of extract from HeLa cells using Histone H3.1 (Ab-10) antibody (E021137, Lane 1 and 2) and Histone H3.1 (phospho-Ser10) antibody (E011184, Lane 3 and 4).