Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 129 (I-L-SP-R-R) (CKRREILSRRPSYR).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16220
Applications:
IHC: 1:50~1:100 ELISA: 1:40000
Alter Name:
CREB1, cAMP response element-binding protein
Specificity Sensitivity:
CREB (phospho-Ser129) antibody detects endogenous levels of CREB only when phosphorylated at serine 129.
References:
Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol; 26: 4934-4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol; 66: 1536-1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003)Arterioscler. Thromb. Vasc. Biol; 23: 1364.
All Sites:
Human: Ser129; Mouse: Ser129; Rat: Ser129
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (phospho-Ser129) antibody.