• Catalog Number:

  E1A8138A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MER/SKY around the phosphorylation site of tyrosine 749/681 (K-I-YP-S-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q12866/Q06418

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  C-mer; EC 2.7.10.1; kinase Mer; MERK; MERTK; proto-oncogene tyrosine-protein kinase MER precursor; receptor tyrosine kinase MerTK

• Specificity Sensitivity:

  MER/SKY (Phospho-Tyr749/681) antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681.

• References:

  Graham D.K., Cell Growth Differ. 5:647-657(1994). Gal A., Nat. Genet. 26:270-271(2000). Hillier L.W., Nature 434:724-731(2005).

• All Sites:

  Human: Tyr749/681; Mouse: Tyr744/671; Rat: Tyr744/671

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho- Tyr749/ 681) antibody. Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MER/SKY (Phospho- Tyr749/681) antibody.