Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 58 (L-P-TP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P01106
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000 IP: Various Dilution
Specificity Sensitivity:
Myc (phospho-Thr58) antibody detects endogenous levels of Myc only when phosphorylated at threonine 58.
References:
Jin Z, et al. (2004) J Biol Chem. 279(38): 40209-40219.
Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101(24): 9085-9090.
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho-Thr58) antibody.
Western blot analysis of extracts from ovary cancer cells using Myc (phospho-Thr58) antibody.