• Catalog Number:

  E1A7028A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine177 (P-F-YP-V-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P11274

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  BCR, BCR protein, BCR1, Breakpoint cluster region protein

• Specificity Sensitivity:

  Bcr (phospho-Tyr177) antibody detects endogenous levels of Bcr only when phosphorylated at tyrosine 177.

• References:

  ML Campbell, Cell Growth Differ., Jul 1993; 4: 581. Asok Mukhopadhyay, J. Biol. Chem., Aug 2002; 277: 30622 - 30628. Jeanne Wilson-Rawls, Cancer Res., Aug 1996; 56: 3426 - 3430. Lori Rink, Blood, Apr 2007; 10.1182.

• All Sites:

  Human: Tyr177; Rat: Tyr72

Western blot analysis of extracts from K562 cells, using Bcr (phospho-Tyr177) antibody (Line 1 and 2).