Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of serine 1179 (S-F-SP-L-Q).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29474
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Other Names:
Constitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS
Specificity Sensitivity:
eNOS (Ab-1179) antibody detects endogenous levels of total eNOS protein.
References:
OLIVIER FERON. Ann. N.Y. Acad. Sci., Jun 1999; 874: 11.
Siripan Phattanarudee. FASEB J, Mar 2006; 20: A1387.
Sci. STKE, Sep 2004; 2004: tw318.
Peptide - +
Western blot analysis of extracts from K562 cells, COLO205 cells and A549 cells, using eNOS (Ab-1179) antibody.
Immunofluorescence analysis of HeLa cells, using eNOS (Ab-1179) antibody.