• Catalog Number:

  E1A7138A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10721

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  CD117, Mast/stem cell growth factor receptor precursor, SCFR, SL, c-kit, kinase Kit

• Specificity Sensitivity:

  c-Kit (phospho-Tyr721) antibody detects endogenous levels of c-Kit only when phosphorylated at tyrosine 721.

• References:

  G. Sakuntala Warshamana-Greene Mol, et al. (2004) Cancer Ther. 3: 527-536. Y Kubota, et al. (1998) Cell Growth Differ. 9: 247-256. Pramod Pandey, et al. (1995) Cancer Res. 55: 4000-4003.  Nancy K. Pryer, Clin. et al. (2003) Cancer Res. 9: 5729-5734.

• All Sites:

  Human: Tyr721; Mouse: Tyr719; Rat: Tyr722

Western blot analysis of extracts from MO7e cells untreated or treated with SCF (50 ng/ml for 2 mins), using c-Kit (Ab-721) antibody (Line 1 and 2) and c-Kit (phospho-Tyr721) antibody (Line 3 and 4).