• Catalog Number:

  E1A1224A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PI3-kinase p85-subunit α/γ around the phosphorylationsite of tyrosine 467/199 (R-L-YP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27986/Q92569

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha

• Specificity Sensitivity:

  PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody detects endogenous levels of PI3-kinase p85-subunit α/γ only when phosphorylated at tyrosine 467/199.

• References:

  Skolnik E.Y., Cell 65:83-90(1991). Antonetti D.A., Mol. Cell. Biol. 16:2195-2203(1996). Van Horn D.J., J. Biol. Chem. 269:29-32(1994).

• All Sites:

  Human: Tyr467/199; Mouse: Tyr467/199; Rat: Tyr467/199

P-peptide - + Western blot analysis of extracts from COS7 cells, treated with H2O2 (100uM, 30mins), using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody. Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody.