Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GSK3β around the phosphorylation site of serine 9.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49841
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000 IP: Various Dilution
Specificity Sensitivity:
GSK3β (Ab-9) antibody detects endogenous levels of total GSK3β protein.
References:
Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
Cross D. A, et al. (1995) Nature. 378: 785-789.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3β (Ab-9) antibody (#B7098).
Western blot analysis of extracts using GSK3β (Ab-9) antibody (#B7098).