• Catalog Number:

  E1A1194A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Casein Kinase I α around the phosphorylation site of threonine 321 (A-Q-TP-P-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P48729

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  CK1-alpha, CKI-alpha, CSNK1A1, Casein kinase I, alpha isoform, EC 2.7.11.1, KC1A, kinase

• Specificity Sensitivity:

  Casein Kinase I α (Phospho-Tyr321) antibody detects endogenous levels of Casein Kinase I α only when phosphorylated at threonine 321.

• References:

  Tapia C., FEBS Lett. 349:307-312(1994). Fish K.J., J. Biol. Chem. 270:14875-14883(1995). Liu C., l 108:837-847(2002).

• All Sites:

  Human: Thr321; Mouse: Thr321; Rat: Thr321

Western blot analysis of extracts from HuvEc cells, using Casein Kinase I α (Phospho-Tyr321) antibody.