• Catalog Number:

  E1A7010B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of Serine 83.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  Q99683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  ASK1(Ab-83) Antibody detects endogenous levels of ASK1 protein around Serine 83.

• References:

  Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using ASK1(Ab-83) Antibody (#B7010). Left: Untreated; Right: Treated with synthesized peptide

Western blot analysis of extracts from MDA-MB- 435 cells. Line1: Using ASK1(Ab-83) Antibody (#B7010); Line2: The same antibody preincubated with synthesized peptide.