• Catalog Number:

  E1A1193A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD227/MUC1 around the phosphorylation site of tyrosine1229 (S-P-YP-E-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P15941

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227; EMA; Episialin; H23AG; MUC-1; mucin 1; Mucin 1 precursor; mucin 1, transmembrane; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

• Specificity Sensitivity:

  CD227/MUC1 (Phospho-Tyr1229) antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.

• References:

  Lan M.S., J. Biol. Chem. 265:15294-15299(1990). Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990). Gendler S.J., J. Biol. Chem. 265:15286-15293(1990).

• All Sites:

  Human: Tyr1229

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody. Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using CD227/MUC1 (Phospho-Tyr1229) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) antibody.