Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human SYK around the phosphorylation site of tyrosine 352.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
SYK (Phospho-Tyr352) antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.
References:
Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994). Law C.-L., J. Biol. Chem. 269:12310-12319(1994).
Law C.-L., J. Biol. Chem. 269:12310-12319(1994).
All Sites:
Human: Tyr352; Mouse: Tyr346; Rat: Tyr346
Western blot analysis of extracts from HepG2 cells, using SYK (Phospho-Tyr352) antibody.