Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 358.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P01106
Applications:
IHC: 1:50~1:100 ELISA: 1:20000
IP: Various Dilution
Specificity Sensitivity:
Myc (phospho-Thr358) antibody detects endogenous levels of Myc only when phosphorylated at threonine 358.
References:
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho-Thr358) antibody.