Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PIP5K around the phosphorylation site of serine 307 (S-A-SP-I-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PIP5K (Phospho-Ser307) antibody detects endogenous levels of PIP5K only when phosphorylated at serine 307.
References:
Ota T., Nat. Genet. 36:40-45(2004).
Nagase T., DNA Res. 6:63-70(1999).
Olsen J.V., Cell 127:635-648(2006).
All Sites:
Human: Ser307; Mouse: Ser318
P-peptide - +
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PIP5K (Phospho-Ser307) antibody.
Immunofluorescence analysis of HeLa cells, using S PIP5K (Phospho-Ser307) antibody.