• Catalog Number:

  E1A1151A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKK-γaround the phosphorylation site of serine 85 (Q-A-SP-Q-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q9Y6K9

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  FIP-3, FIP3, I-kappa-B kinase gamma, IKBKG, IKK-gamma, IKKAP1, IKKG, IkB kinase gamma subunit, IkB kinase-associated protein 1, Inhibitor of nuclear factor kappa-B kinase gamma subunit, NEMO, NF-kappaB essential modifier, NF-kappaB essential modulator, mFIP-3

• Specificity Sensitivity:

  IKK-γ (Phospho-Ser85) antibody detects endogenous levels of IKK-γ only when phosphorylated at serine 85.

• References:

  Li Y., Proc. Natl. Acad. Sci. U.S.A. 96:1042-1047(1999). Jin D.-Y., J. Biomed. Sci. 6:115-120(1999). Rothwarf D.M., Nature 395:297-300(1998).

• All Sites:

  Human: Ser85; Mouse: Ser85

Western blot analysis of extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), using IKK-γ (Phospho-Ser85) antibody.