• Catalog Number:

  E1A7015B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of Threonine 71 or 53.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15336

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  ATF-2(Ab-71or 53) Antibody detects endogenous levels of ATF-2 protein around Threonine 71 or 53.

• References:

  Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465. Waetzig G H, et al. (2002) J Immunol. 168(10): 5342-5351. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.

Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using ATF-2 (Ab-71or 53) Antibody (#B7015); Right: The same antibody preincubated with synthesized peptide.

Western blot analysis of extracts from HeLa cells. Line1: Using ATF-2(Ab-71or 53) Antibody (#B7015); Line2: Using the same antibody preincubated with synthesized peptide.