• Catalog Number:

  E1A1148A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FANCA around the phosphorylation site of serine 1149 (D-P-SP-L-M).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O15360

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  FA; Fa; FA-H; FA1; FAA; FACA; FAH; FANCH; Fanconi anemia group A protein; Fanconi anemia, complementation group A

• Specificity Sensitivity:

  FANCA (Phospho-Ser1149) antibody detects endogenous levels of FANCA only when phosphorylated at serine 1149.

• References:

  Lo Ten Foe J.R., Nat. Genet. 14:320-323(1996). Ianzano L., Genomics 41:309-314(1997). Ricke D.O., Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser1149

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using FANCA (Phospho-Ser1149) antibody. Western blot analysis of extracts from HELA cells treated with IGF (100ng/ml, 10mins) and HUVEC cells treated with EGF (200ng/ml, 30mins), using FANCA (Phospho-Ser1149) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using FANCA (Phospho-Ser1149) antibody.