Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 262 (I-G-SP-T-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Tau (phospho-Ser262) antibody detects endogenous levels of Tau only when phosphorylated at serine 262.
References:
Timm T, et al. (2003) EMBO J; 22(19): 5090-5101.
Western blot analysis of extracts from mouse brain tissue using Tau (Ab-262) antibody (E021100, Lane 1 and 2) and Tau (phospho-Ser262) antibody ( E011111, Lane 3 and 4).