• Catalog Number:

  E1A1123A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TAK1 around the phosphorylation site of threonine 184 (I-Q-TP-H-M).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O43318

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  EC 2.7.11.25, M3K7, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, TGF-beta- activated kinase 1, Transforming growth factor-beta-activated kinase 1, kinase TAK1

• Specificity Sensitivity:

  TAK1 (Phospho-Thr184) antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.

• References:

  Sakurai H., Biochem. Biophys. Res. Commun. 243:545-549(1998). Mungall A.J., Nature 425:805-811(2003). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Thr184; Mouse: Thr184; Rat: Thr184

Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody.