• Catalog Number:

  E1A1121A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ROMK/Kir1.1 around the phosphorylation site of serine 44/25 (L-V-SP-K-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P48048

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  ATP-regulated potassium channel ROM-K, ATP-sensitive inward rectifier potassium channel 1, IRK1, KAB-1, KCNJ1, Kir1.1, Potassium channel, inwardly rectifying, subfamily J, member 1, ROMK1

• Specificity Sensitivity:

  ROMK/Kir1.1 (Phospho-Ser44/25) antibody detects endogenous levels of ROMK/Kir1.1 only when phosphorylated at serine 44/25.

• References:

  DW Lundgren, Proc. Soc. Exp. Biol. Med., Oct 1997; 216: 57. Dana Yoo, J. Biol. Chem., Jun 2003; 278: 23066 - 23075. W. S. Lee, Am J Physiol Renal Physiol, Jun 1995; 268: 1124.

• All Sites:

  Human: Ser44; Mouse: Ser25; Rat: Ser44

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody. Immunofluorescence analysis of A549 cells, using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.

ROMK/Kir1.1 (Phospho-Ser44/25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.