Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human IGFBP-3 around the phosphorylation site of serine 183 (K-D-SP-Q-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mous
SwissProtNo:
P17936
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
IBP-3, IBP3, IGF-binding protein 3, IGFBP3, Insulin-like growth factor binding protein 3 precursor
Specificity Sensitivity:
IGFBP-3 (Ab-183) antibody detects endogenous levels of total IGFBP-3 protein.
References:
Wood W.I., Mol. Endocrinol. 2:1176-1185(1988).
Cubbage M.L., J. Biol. Chem. 265:12642-12649(1990).
Thweatt R., DNA Seq. 4:43-46(1993).
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using IGFBP-3 (Ab-183) antibody.
Western blot analysis of extracts from HUVEC cells, using IGFBP-3 (Ab-183) antibody.