• Catalog Number:

  E1A1120A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RapGEF1 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13905

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  C3G, CRK SH3-binding GNRP, GRF2, RPGF1, Rap guanine nucleotide exchange factor 1, guanine nucleotide-releasing factor 2

• Specificity Sensitivity:

  RapGEF1 (Phospho-Tyr504) antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine 504.

• References:

  Tanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994). Knudsen B., J. Biol. Chem. 269:32781-32787(1994). Matsuda M., J. Biol. Chem. 271:14468-14472(1996).

• All Sites:

  Human: Tyr504; Mouse: Tyr514

Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using RapGEF1 (Phospho-Tyr504) antibody.