Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Myogenic Factor 5 around the phosphorylation site of serine 49 (Q-G-SP-D-E).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P13349
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
MYF5, myogenic factor 5
Specificity Sensitivity:
Myogenic Factor 5 (Phospho-Ser49) antibody detects endogenous levels of Myogenic Factor 5 only when phosphorylated at serine 49.
References:
Braun T., EMBO J. 8:701-709(1989).
Thompson D.B., Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
All Sites:
Human: Ser49; Mouse: Ser49
Western blot analysis of extracts from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) antibody.