• Catalog Number:

  E1A1104A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p100 around the phosphorylation site of serine 872 (S-Q-SP-V-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q00653

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10, Lyt10, NFKB2, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10

• Specificity Sensitivity:

  NF-κB p100 (Phospho-Ser872) antibody detects endogenous levels of NF-κB p100 only when phosphorylated at serine 872.

• References:

  Schmid R.M., Nature 352:733-736(1991). Bours V., Mol. Cell. Biol. 12:685-695(1992). Thakur S., Oncogene 9:2335-2344(1994).

• All Sites:

  Human: Ser872; Mouse: Ser871

Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using NF-κB p100 (Phospho-Ser872) antibody.