• Catalog Number:

  E011174-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G- SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13131 P54646

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at serine 485 or serine 491.

• References:

  Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).

Western blot analysis of extract from HeLa cells untreated or treated with heat shock (30min), using AMPK1/AMPK2 (phospho-Ser485/Ser491) antibody (E011174).