• Catalog Number:

  E1A1102A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of threonine 558 (Y-K-TP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P26038

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  MOES, MSN, Membrane-organizing extension spike protein

• Specificity Sensitivity:

  Moesin/Ezrin/Radixin (Phospho-Thr558) antibody detects endogenous levels of Moesin/Ezrin/Radixin only when phosphorylated at threonine 558.

• References:

  Lankes W.T., Proc. Natl. Acad. Sci. U.S.A. 88:8297-8301(1991). Ross M.T., Nature 434:325-337(2005). Gevaert K., Nat. Biotechnol. 21:566-569(2003). Kim S.C., Mol. Cell 23:607-618(2006).

• All Sites:

  Human: Thr558; Mouse: Thr558; Rat: Thr558

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using Moesin/Ezrin/Radixin (Phospho-Thr558) antibody. Western blot analysis of extracts NIH/3T3 Jurkat cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) antibody.