Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human LKB1 around the phosphorylation site of serine 334 (W-R-SP-M-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q15831
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity Sensitivity:
LKB1 (phospho-Ser334) antibody detects endogenous levels of LKB1 only when phosphorylated at serine 334.
References:
Shaw, R.J. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.
Woods, A. et al. (2003) Curr. Biol. 13, 2004-2008.
Karuman P, et al. (2001) Mol Cell 7, 1307-19
Nezu, J. et al. (1999) Biochem. Biophys. Res. Commun. 261, 750-755
Hemminki, A. et al. (1998) Nature 391, 184-187
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using LKB1 (phospho-Ser334) antibody (E012045).