• Catalog Number:

  E1A7026B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  This antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of Threonine 56.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  BCL-2(Ab-56) Antibody detects endogenous levels of total BCL-2 protein.

• References:

  Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using BCL-2(Ab-56) Antibody(#B7026). Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis of extracts from MCF7 cells treated with etoposide. Line1: Using BCL-2(Ab-56) Antibody (#B7026); Line2: The same antibody pre- incubated with synthesized peptide.