• Catalog Number:

  E1A1064A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IL-4R/CD124 around the phosphorylation site of tyrosine 497 (P-A-YP-R-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P24394

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  582J2.1, CD124 antigen, IL-4R-alpha, IL4RA, Interleukin-4 receptor alpha chain precursor

• Specificity Sensitivity:

  IL-4R/CD124 (Phospho-Tyr497) antibody detects endogenous levels of IL-4R/CD124 only when phosphorylated at tyrosine 497.

• References:

  Idzerda R.L., J. Exp. Med. 171:861-873(1990). Galizzi J.-P., Int. Immunol. 2:669-675(1990). Loftus B.J., Genomics 60:295-308(1999). Kruse S., Int. Immunol. 11:1965-1970(1999).

• All Sites:

  Human: Tyr497; Mouse: Tyr500

P-peptide - + Western blot analysis of extracts from 293 cells, using IL-4R/CD124 (Phospho-Tyr497) antibody. Immunofluorescence analysis of HeLa cells, using IL-4R/CD124 (Phospho-Tyr497) antibody.